Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs

Metabolomics is a technique that allows for the evaluation of entire extractable chemical profile plant, example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due drought. Metabolomic analysis dependent upon efficiency extraction protocol. Currently, there are two common procedures widely in metabolomic experiments, extract from tissue processed liquid nitrogen or lyophilised tissues. Here, we evaluated non-targeted metabolomics show lyophilisation stabilise maize (Zea mays) metabolome, increasing throughput compared more traditional processing nitrogen. Then, applied approach explore effect drought metabolome HRMS approach. revealed differences mature having undergone three conditions imposed at critical development stages (three-leaf stage grain-fill stage); moreover, this difference was observed across types (kernel inner cob/pith). It shown under ideal conditions, biochemical make-up different. However, response dominates metabolic profile. Drought-related metabolites known other systems have been identified has potential novel drought-stress indicators our system.